6AXL

Crystal structure of Fab317 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	82.663, 65.342, 99.043
Unit cell angles	90.00, 106.40, 90.00

Refinement procedure

Resolution	40.930 - 2.400
R-factor	0.178
Rwork	0.175
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	homology model
RMSD bond length	0.002
RMSD bond angle	0.591
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.930	2.440
High resolution limit [Å]	2.400	2.400
Rpim	0.079	0.430
Number of reflections	38939
<I/σ(I)>	11.2
Completeness [%]	97.8
Redundancy	6.1
CC(1/2)	0.920	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.1M Sodium Citrate pH4.0 1M Lithium Chloride 20% PEG6000