6AXK
Crystal structure of Fab311 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-27 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 66.117, 44.729, 186.079 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.520 - 2.103 |
R-factor | 0.2053 |
Rwork | 0.204 |
R-free | 0.23560 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | homology model |
RMSD bond length | 0.002 |
RMSD bond angle | 0.663 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.520 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.077 | 0.500 |
Number of reflections | 32026 | |
<I/σ(I)> | 11 | |
Completeness [%] | 98.1 | |
Redundancy | 5.1 | |
CC(1/2) | 0.821 | 0.465 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1M MES pH6.0 36% PEG400 5% PEG3000 |