6AW0
Crystal structure of CEACAM3 L44Q
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-07-14 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 92.810, 92.810, 155.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 79.750 - 2.430 |
| R-factor | 0.2225 |
| Rwork | 0.221 |
| R-free | 0.24356 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qsq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.333 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.750 | 2.620 |
| High resolution limit [Å] | 2.430 | 2.430 |
| Rmerge | 0.243 | 1.595 |
| Rpim | 0.137 | 0.913 |
| Number of reflections | 13227 | |
| <I/σ(I)> | 8.2 | 1.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.7 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | 0.8M Ammonium Sulphate, 1% PEG 3350, 0.1M Bis Tris pH 5.5 |
