6AVT
STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING D4TTP AT PH 9.5 WITH CROSS-LINKING TO FIRST BASE TEMPLATE OVERHANG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-11 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9181 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.197, 133.504, 140.129 |
| Unit cell angles | 90.00, 97.49, 90.00 |
Refinement procedure
| Resolution | 25.892 - 2.603 |
| R-factor | 0.1795 |
| Rwork | 0.179 |
| R-free | 0.21130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 6AMO |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.422 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.11.1 2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.600 | 2.600 |
| Rmerge | 0.085 | 0.043 | 0.701 |
| Number of reflections | 99750 | 9755 | 9865 |
| <I/σ(I)> | 18.1 | 22.5 | 1.8 |
| Completeness [%] | 99.3 | 95.6 | 98.7 |
| Redundancy | 6.6 | 7.2 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 277 | PEG 8000, NaCl, CHES (N-Cyclohexyl-2-aminoethanesulfonic acid), TRIS, MgCl2, d4T triphosphate |
