6AVJ
Crystal structure of human Mitochondrial inner NEET protein (MiNT)/CISD3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-20 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.406, 52.250, 78.951 |
| Unit cell angles | 90.00, 108.67, 90.00 |
Refinement procedure
| Resolution | 34.240 - 1.900 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.19770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tbn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.766 |
| Data reduction software | XPREP |
| Data scaling software | APEX |
| Phasing software | PHASER |
| Refinement software | PHENIX (2.7.16) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.240 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.111 | 0.489 |
| Rpim | 0.042 | 0.323 |
| Number of reflections | 27910 | 2593 |
| <I/σ(I)> | 11.9 | 2.3 |
| Completeness [%] | 99.4 | 93.58 |
| Redundancy | 7.1 | 3.05 |
| CC(1/2) | 0.995 | 0.637 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 100 mM bis-tris propane, 2.5 M ammonium sulfate, 3% v/v polyethylene glycol 400 |
