6AU8

1.8 angstrom crystal structure of the human Bag6-NLS & TRC35 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-02-14
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9200, 0.8154
Spacegroup name	P 21 21 21
Unit cell lengths	41.660, 84.560, 102.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.090 - 1.800
R-factor	0.1615
Rwork	0.159
R-free	0.20180
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.167
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.090	39.090	1.850
High resolution limit [Å]	1.800	8.050	1.800
Rmerge	0.080	0.030	0.708
Rmeas	0.085	0.032	0.747
Total number of observations	323983
Number of reflections	34348	454	2488
<I/σ(I)>	19.15	55.22	3.46
Completeness [%]	99.9	99.1	100
Redundancy	9.432	8.427	9.795
CC(1/2)	0.999	0.999	0.933

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	20% PEG-3350, 0.2 M DL-malic acid, pH 7.0