6ATX

Crystal structure of Physcomitrella patens KAI2-like C

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-08-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 43
Unit cell lengths	46.630, 46.630, 140.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.630 - 2.740
R-factor	0.179600563238
Rwork	0.178
R-free	0.21901
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ih1
RMSD bond length	0.003
RMSD bond angle	0.596
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.630	2.840
High resolution limit [Å]	2.740	2.740
Rmerge	0.220	0.834
Rpim	0.062	0.296
Number of reflections	7881
<I/σ(I)>	12.35
Completeness [%]	99.9
Redundancy	13.7
CC(1/2)	0.994	0.504

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.2 M ammonium sulfate, 0.2 M Bis-Tris, pH 6.5