6ATV
The molecular mechanisms by which NS1 of the 1918 Spanish influenza A virus hijack host protein-protein interactions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 113 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-12-02 |
| Detector | RIGAKU |
| Wavelength(s) | 1.45 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 39.840, 39.840, 171.951 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.500 - 1.751 |
| R-factor | 0.1857 |
| Rwork | 0.184 |
| R-free | 0.20530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ul6 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.781 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.500 |
| High resolution limit [Å] | 1.751 |
| Number of reflections | 8437 |
| <I/σ(I)> | 16.8 |
| Completeness [%] | 94.6 |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M sodium acetate (pH 4.6), 30% PEG 2000, and 0.2 M ammonium sulfate |
