6ATQ
Crystal structure of apo-hGSTA1-1 exhibiting a new conformation of C-terminal helix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-23 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.591, 90.692, 51.250 |
| Unit cell angles | 90.00, 92.53, 90.00 |
Refinement procedure
| Resolution | 34.917 - 2.000 |
| R-factor | 0.171 |
| Rwork | 0.169 |
| R-free | 0.23480 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1guh |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.986 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.120 | |
| Number of reflections | 29807 | 2624 |
| <I/σ(I)> | 13.48 | |
| Completeness [%] | 96.8 | 85.8 |
| Redundancy | 6.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 2000 MME, 25% (w/v), 0.1 M MES, 6.5 |
