6ATI
HLA-DRB1*1402 in complex with Vimentin-64Cit59-71
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-05 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | .95370 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.270, 76.870, 94.910 |
| Unit cell angles | 90.00, 109.68, 90.00 |
Refinement procedure
| Resolution | 33.629 - 1.980 |
| R-factor | 0.192 |
| Rwork | 0.190 |
| R-free | 0.23330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mcy |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.885 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.629 | 2.090 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rpim | 0.056 | 0.195 |
| Number of reflections | 63444 | 9163 |
| <I/σ(I)> | 9.1 | 4.3 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 294 | 26% PEG 3350, 0.2M Potassium Nitrate, 0.1M Bis-Tris-Propane pH 7.3 |
