6ASY
BiP-ATP2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-08 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 75.829, 75.752, 78.791 |
| Unit cell angles | 62.05, 62.23, 73.42 |
Refinement procedure
| Resolution | 39.087 - 1.850 |
| R-factor | 0.16 |
| Rwork | 0.159 |
| R-free | 0.18920 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.202 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.850 |
| Rmerge | 0.070 |
| Number of reflections | 113887 |
| <I/σ(I)> | 29.3 |
| Completeness [%] | 97.8 |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 18-22% PEG 1000, 0.1 M phosphate citrate (pH 4.2-7.0), and 0.2 M Li2SO4 |
