6ASG

Crystal structure of Thermus thermophilus RNA polymerase core enzyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-13
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97926
Spacegroup name	H 3
Unit cell lengths	280.694, 280.694, 184.964
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.492 - 3.800
R-factor	0.2312
Rwork	0.229
R-free	0.27820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gzy
RMSD bond length	0.003
RMSD bond angle	0.658
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.870
High resolution limit [Å]	3.800	10.290	3.800
Rmerge	0.245	0.065	0.704
Rmeas	0.275	0.072	0.830
Rpim	0.124	0.030	0.430
Total number of observations	295018
Number of reflections	61395	3093	2858
<I/σ(I)>	5.2
Completeness [%]	99.2	99.7	92.3
Redundancy	4.8	5.5	3.2
CC(1/2)		0.989	0.418

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	0.02M Magnesium chloride hexahydrate, 0.1M HEPES pH 7.5, 22% Poly(acrylic acid sodium salt) 5,100