6ASE
KRAS mutant-A59G in GDP-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-30 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.434, 47.428, 89.703 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.588 - 1.554 |
| R-factor | 0.1974 |
| Rwork | 0.196 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4obe |
| RMSD bond length | 0.036 |
| RMSD bond angle | 0.896 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.580 |
| High resolution limit [Å] | 1.550 | 4.210 | 1.550 |
| Rmerge | 0.088 | 0.041 | 0.815 |
| Rmeas | 0.095 | 0.044 | 0.961 |
| Rpim | 0.035 | 0.016 | 0.493 |
| Total number of observations | 142276 | ||
| Number of reflections | 21284 | 1213 | 836 |
| <I/σ(I)> | 9.1 | ||
| Completeness [%] | 97.2 | 99.2 | 78.2 |
| Redundancy | 6.7 | 7.1 | 2.9 |
| CC(1/2) | 0.998 | 0.394 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 0.15 M Cesium chloride, 15 % PEG 3350, 40 mM MgCl2 |
