6AS9
Filamentous Assembly of Green Fluorescent Protein Supported by a C-terminal fusion of 18-residues, viewed in space group P212121 form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-07-22 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.100, 54.520, 85.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.960 - 1.750 |
R-factor | 0.1572 |
Rwork | 0.156 |
R-free | 0.18310 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.100 |
Data reduction software | XDS (20160517) |
Data scaling software | XSCALE (20160517) |
Phasing software | PHASER (2.6.1) |
Refinement software | BUSTER (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 45.960 | 45.960 | 1.800 |
High resolution limit [Å] | 1.750 | 7.840 | 1.750 |
Rmerge | 0.117 | 0.052 | 0.585 |
Rmeas | 0.126 | 0.057 | 0.630 |
Total number of observations | 175062 | ||
Number of reflections | 24417 | 326 | 1743 |
<I/σ(I)> | 14.07 | 28.9 | 3.76 |
Completeness [%] | 99.4 | 99.7 | 98.5 |
Redundancy | 7.17 | 6.132 | 7.009 |
CC(1/2) | 0.996 | 0.996 | 0.857 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 0.1 M sodium acetate, pH 5.0 and 65% (v/v) 2-methyl-2,4-pentanediol |