6APO
Anti-Marburgvirus Nucleoprotein Single Domain Antibody A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97917 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.597, 49.362, 58.814 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.810 - 1.168 |
| R-factor | 0.149 |
| Rwork | 0.148 |
| R-free | 0.17280 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yc7 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.967 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.600 | 1.230 |
| High resolution limit [Å] | 1.168 | 1.170 |
| Rpim | 0.028 | 0.198 |
| Number of reflections | 40045 | 4261 |
| <I/σ(I)> | 17.6 | 3.4 |
| Completeness [%] | 95.7 | 71.3 |
| Redundancy | 6.6 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 25% polyethylene glycol 1000, 0.1M Tris-HCl pH 8.5 |
