6AP0

Crystal structure of human FLASH N-terminal domain C54S/C83A (Crystal form 2)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	C 1 2 1
Unit cell lengths	63.478, 44.346, 68.433
Unit cell angles	90.00, 98.65, 90.00

Refinement procedure

Resolution	36.216 - 2.581
R-factor	0.2156
Rwork	0.214
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ano
RMSD bond length	0.003
RMSD bond angle	0.534
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.216	2.740
High resolution limit [Å]	2.580	2.580
Rmerge	0.079	0.467
Number of reflections	11542	1848
<I/σ(I)>	13.42	3.82
Completeness [%]	99.6	99.2
Redundancy	3.8	3.7
CC(1/2)	0.998	0.898

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM sodium formate, 15% (w/v) PEG 3350, 3% (v/v) 1,6-hexanediol