6AOP

Crystal structure of the A/Brisbane/10/2007 (H3N2) influenza virus hemagglutinin L194P mutant apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-29
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	H 3 2
Unit cell lengths	100.491, 100.491, 383.319
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.300
R-factor	0.19881
Rwork	0.197
R-free	0.23230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o5n
RMSD bond length	0.011
RMSD bond angle	1.530
Data scaling software	HKL-2000 (712)
Phasing software	PHASER (2.5.6)
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.370
High resolution limit [Å]	2.300	2.300
Rpim	0.030	0.220
Number of reflections	34068	2928
<I/σ(I)>	31.7	1.6
Completeness [%]	99.6	95.9
Redundancy	16.2	9
CC(1/2)	1.000	0.790

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	293	0.1 M CAPS pH 10.5 and 29% PEG 400