6AO9

Crystal structure of monomeric guanylyl cyclase domain of RhoGC fusion protein from the aquatic fungus Blastocladiella emersonii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 2 21 21
Unit cell lengths	34.460, 65.570, 90.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.684 - 1.130
R-factor	0.1879
Rwork	0.188
R-free	0.19540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wz1
RMSD bond length	0.005
RMSD bond angle	0.773
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.190
High resolution limit [Å]	1.130	1.130
Rmerge	0.077	1.000
Number of reflections	77710
<I/σ(I)>	15.2	2.2
Completeness [%]	99.9	99.7
Redundancy	12.6	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	25% PEG3350, 100 mM BIS-TRIS, pH 5.5, 200 mM NaCl