6ANA

LL2 Fab in complex with anti-Kappa VHH domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-09-18
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 1
Unit cell lengths	38.925, 63.682, 68.417
Unit cell angles	114.28, 96.14, 91.92

Refinement procedure

Resolution	38.559 - 1.700
R-factor	0.2154
Rwork	0.215
R-free	0.23650
RMSD bond length	0.004
RMSD bond angle	0.824
Data reduction software	XPREP
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.559	1.720
High resolution limit [Å]	1.700	1.700
Rmerge	0.051	0.362
Rpim	0.036	0.258
Number of reflections	58574
<I/σ(I)>	15.8
Completeness [%]	89.6
Redundancy	2.7
CC(1/2)	0.998	0.765

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	1M lithium chloride 0.1M MES, pH 6.0