6AMJ

CAT192 Fab Wild Type

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E+ SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-24
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5419
Spacegroup name	P 1 21 1
Unit cell lengths	55.950, 83.350, 104.910
Unit cell angles	90.00, 104.77, 90.00

Refinement procedure

Resolution	30.713 - 2.490
R-factor	0.209
Rwork	0.206
R-free	0.26180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gsz 3skj
RMSD bond length	0.003
RMSD bond angle	0.473
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.713	2.620
High resolution limit [Å]	2.490	2.490
Rmerge		0.396
Number of reflections	32712	4715
<I/σ(I)>		2.3
Completeness [%]		98.8
Redundancy		2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	12% PEG 8K, 0.1M Sodium cacodylate pH 6.0, 0.2M MgCl2