6AM3

Regulator of G protein signaling (RGS) 17 in complex with Ca2+

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	115
Detector technology	PIXEL
Collection date	2017-03-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.078
Spacegroup name	P 1 21 1
Unit cell lengths	39.571, 59.295, 65.337
Unit cell angles	90.00, 100.60, 90.00

Refinement procedure

Resolution	30.000 - 1.530
R-factor	0.2043
Rwork	0.203
R-free	0.22470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1zv4
RMSD bond length	0.009
RMSD bond angle	1.310
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.560
High resolution limit [Å]	1.530	4.150	1.530
Rmerge	0.132	0.056	0.665
Rmeas	0.146	0.060	0.846
Rpim	0.061	0.022	0.517
Total number of observations	190012
Number of reflections	39255	2321	921
<I/σ(I)>	4.6
Completeness [%]	87.5	99.9	41.4
Redundancy	4.8	7.2	1.7
CC(1/2)		0.997	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.2 M calcium chloride dihydrate, 0.1 M MES, pH 6.0, 22% PEG3350