6ALX

Structure of F. tularensis MglA-SspA solved in the presence of polyP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-08-08
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	67.253, 114.298, 141.651
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	60.753 - 3.300
R-factor	0.2177
Rwork	0.209
R-free	0.29440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5u56
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.754	3.480
High resolution limit [Å]	3.300	3.300
Rmeas	0.117	0.643
Rpim	0.062	0.280
Number of reflections	16079
<I/σ(I)>	8.1	2.5
Completeness [%]	94.2	100
Redundancy	3.5	3.3
CC(1/2)	0.994	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Peg 3350