6ALE
A V-to-F substitution in SK2 channels causes Ca2+ hypersensitivity and improves locomotion in a C. elegans ALS model
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | CMOS |
| Collection date | 2017-07-12 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 76.653, 66.913, 64.712 |
| Unit cell angles | 90.00, 92.47, 90.00 |
Refinement procedure
| Resolution | 24.193 - 2.500 |
| R-factor | 0.1912 |
| Rwork | 0.188 |
| R-free | 0.25510 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.127 |
| Data reduction software | XPREP |
| Data scaling software | XPREP |
| Phasing software | PHASES |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.720 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 11420 |
| <I/σ(I)> | 13.3 |
| Completeness [%] | 100.0 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293.15 | 0.1 M Sodium Citrate Tribasic dehydrate 0.6 M Ammonium sulfate 1.4 M Lithium sulfate monohydrate |
