6AL5
COMPLEX BETWEEN CD19 (N138Q MUTANT) AND B43 FAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-19 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 64.800, 92.350, 146.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.000 |
| R-factor | 0.19307 |
| Rwork | 0.189 |
| R-free | 0.26302 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5i15 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.178 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.080 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.083 | 0.496 |
| Number of reflections | 18262 | 1285 |
| <I/σ(I)> | 18.4 | 3.6 |
| Completeness [%] | 99.5 | 96.6 |
| Redundancy | 6.4 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES PH 7.5, 23% PEG 8000 |
