6AL2

Crystal structure of E. coli YidC at 2.8 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-20
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 1
Unit cell lengths	42.910, 76.190, 91.950
Unit cell angles	78.03, 82.61, 78.05

Refinement procedure

Resolution	41.842 - 2.800
R-factor	0.2226
Rwork	0.218
R-free	0.27490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wvf
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (2.7.15)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.842	41.842	2.970
High resolution limit [Å]	2.800	8.370	2.800
Rmerge	0.333	0.142	1.361
Rmeas	0.355	0.150	1.450
Total number of observations	228470
Number of reflections	27283	1013	4416
<I/σ(I)>	5.64	17.11	1.34
Completeness [%]	99.8	99	99.8
Redundancy	8.374	9.154	8.575
CC(1/2)	0.978	0.984	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5	293	30% PEG 600, KSCN, Na-acetate