6AKZ
Crystal structure of GlcNAc Inducible Gene 2, GIG2 (DUF1479) from Candida albicans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-04-16 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.652, 54.545, 74.850 |
Unit cell angles | 90.00, 105.61, 90.00 |
Refinement procedure
Resolution | 72.090 - 1.690 |
R-factor | 0.18223 |
Rwork | 0.181 |
R-free | 0.21623 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2csg |
RMSD bond length | 0.019 |
RMSD bond angle | 1.891 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.090 | 1.750 |
High resolution limit [Å] | 1.690 | 1.690 |
Rmerge | 0.100 | 1.001 |
Number of reflections | 52149 | 4894 |
<I/σ(I)> | 17.4 | |
Completeness [%] | 99.0 | |
Redundancy | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 25% PEG 6000, 0.1M Tris pH 8.0, 0.1M MgCl2 |