6AKV

Crystal structure of LysB4, the endolysin from Bacillus cereus-targeting bacteriophage B4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2017-05-29
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	I 41
Unit cell lengths	79.457, 79.457, 77.925
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.092 - 2.400
R-factor	0.1805
Rwork	0.176
R-free	0.22430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vo9
RMSD bond length	0.007
RMSD bond angle	0.785
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.13_2998))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.440
High resolution limit [Å]	2.400	2.400
Rmerge	0.057	0.351
Rmeas	0.063	0.419
Rpim	0.025	0.222
Number of reflections	8863	449
<I/σ(I)>	19.66	2.36
Completeness [%]	91.5	92.4
Redundancy	4.9	2.9
CC(1/2)		0.353

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	287.15	2.0 M ammonium sulfate, 0.1 M Bis-Tris pH 6.5, 2% polyethylene glycol monomethyl ether 550 (PEGMME 550), 8 mM Tris(2-carboxyethyl)phosphine (TCEP)