6AKL

Crystal structure of Striatin3 in complex with SIKE1 Coiled-coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	39.282, 42.382, 91.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.048 - 1.750
R-factor	0.1893
Rwork	0.187
R-free	0.21330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6akk
RMSD bond length	0.007
RMSD bond angle	0.774
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.750	1.750
Rmerge	0.067
Rmeas	0.070
Rpim	0.020	0.376
Number of reflections	16205	798
<I/σ(I)>	28	2
Completeness [%]	99.8	99.4
Redundancy	12.5	11.4
CC(1/2)		0.828

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	35% (v/v) MPD; 0.1 M imidazole pH 7.5