6AK7
Crystal structure of PPM1K-N94K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 293 |
Detector technology | PIXEL |
Collection date | 2017-10-03 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 122.784, 122.784, 61.580 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.445 - 2.605 |
R-factor | 0.1939 |
Rwork | 0.192 |
R-free | 0.22430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4da1 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.500 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 16613 | |
<I/σ(I)> | 0.3 | |
Completeness [%] | 99.6 | |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1M Tris, pH 8.5, 12% PEG 3350, 0.2 M MgCl2, 10 mM Mercaptoethanol |