6AJL

DOCK7 mutant I1836Y complexed with Cdc42

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	194.550, 105.290, 117.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.638 - 3.230
R-factor	0.1883
Rwork	0.184
R-free	0.25870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6aj4
RMSD bond length	0.010
RMSD bond angle	1.242
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.430
High resolution limit [Å]	3.230	3.230
Rmeas	0.180	0.940
Number of reflections	74614	11786
<I/σ(I)>	7.5	1.6
Completeness [%]	99.4	97.7
Redundancy	3.6
CC(1/2)	0.991	0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	293	PEG3350, tri-potassium citrate monohydrate