6AJK
Crystal structure of TFB1M and h45 in homo sapiens
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2016-01-20 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9790 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 122.564, 122.564, 73.067 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.271 - 3.001 |
R-factor | 0.1988 |
Rwork | 0.197 |
R-free | 0.24110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gc5 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.952 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.000 | 40.000 | 3.110 |
High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
Rmerge | 0.252 | 0.099 | 0.819 |
Rmeas | 0.273 | 0.108 | 0.886 |
Rpim | 0.101 | 0.041 | 0.322 |
Number of reflections | 11458 | 1234 | 1136 |
<I/σ(I)> | 2 | ||
Completeness [%] | 98.3 | 96.3 | 99.6 |
Redundancy | 6.2 | 5.6 | 6.5 |
CC(1/2) | 0.994 | 0.781 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293 | 0.1M sodium citrate, pH 5.0, 20% PEG 8000 |