6AIP
Cab2 mutant-H337A complex with phosphopantothenoylcystine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-01-26 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97853 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.097, 112.468, 54.573 |
| Unit cell angles | 90.00, 91.12, 90.00 |
Refinement procedure
| Resolution | 56.230 - 1.990 |
| R-factor | 0.1826 |
| Rwork | 0.181 |
| R-free | 0.21760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p9o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.415 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.230 | 2.030 |
| High resolution limit [Å] | 1.990 | 2.000 |
| Rmerge | 0.110 | 0.604 |
| Rpim | 0.047 | 0.270 |
| Number of reflections | 40420 | 2030 |
| <I/σ(I)> | 16.7 | |
| Completeness [%] | 96.2 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.873 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 287 | 200mM ammonium tartrate, 20% PEG 3350 2mM MnCl2 |
