6AIE
Crystal structure of a new form of RsmD-like RNA methyl transferase from Mycobacterium tuberculosis determined at 1.74 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 95.782, 55.300, 100.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.390 - 1.740 |
| R-factor | 0.21211 |
| Rwork | 0.211 |
| R-free | 0.24260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3p9n |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.890 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.800 | 1.770 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.019 | |
| Number of reflections | 57366 | 3777 |
| <I/σ(I)> | 5.6 | 5.6 |
| Completeness [%] | 98.7 | |
| Redundancy | 3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 2M Potassium acetate, 100mM Tris (PH 7.4) |
