6AI1
Structure of the 328-692 fragment of FlhA (D456V)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-08 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 1.00 |
| Spacegroup name | I 41 |
| Unit cell lengths | 216.790, 216.790, 64.980 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.198 - 3.500 |
| R-factor | 0.1944 |
| Rwork | 0.189 |
| R-free | 0.24120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3a5i |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.239 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.600 | 3.830 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.137 | 0.449 |
| Number of reflections | 19380 | 4609 |
| <I/σ(I)> | 7.6 | 3.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 30% PEG-400, 0.1M HEPES pH 7.5, 0.25M NaCl |
