6AH8
Marine bacterial prolidase with promiscuous organophosphorus hydrolase activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2018-05-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97892 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 178.854, 178.854, 371.586 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.530 - 2.610 |
| Rwork | 0.226 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4zwo |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.995 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.176 | 0.851 |
| Number of reflections | 70044 | 6932 |
| <I/σ(I)> | 14.1 | 2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 9.9 | 9.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 2 M Ammonium sulfate, 5% PEG400, 100 mM MES pH6.5 |
