6AG4

Crystal structure of Ard1 N-terminal acetyltransferase H88A/E127A mutant from Sulfolobus solfataricus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	77
Detector technology	CCD
Collection date	2014-03-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	42.335, 53.148, 74.505
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.967 - 2.256
R-factor	0.1692
Rwork	0.163
R-free	0.22850
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4r3k
RMSD bond length	0.007
RMSD bond angle	1.022
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.360
High resolution limit [Å]	2.250	2.250
Number of reflections	8167
<I/σ(I)>	24.6
Completeness [%]	98.1
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	283	0.75M ammonium sulfate, 0.1M sodium acetate trihydrate, pH 4.2, 0.1M lithium sulfate