6AF0
Structure of Ctr9, Paf1 and Cdc73 ternary complex from Myceliophthora thermophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2018-07-07 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9789 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 99.867, 237.789, 62.737 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 118.890 - 2.880 |
| R-factor | 0.24829 |
| Rwork | 0.245 |
| R-free | 0.30929 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.362 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 118.890 | 2.950 |
| High resolution limit [Å] | 2.880 | 2.900 |
| Rmerge | 0.075 | 1.230 |
| Number of reflections | 34480 | 1718 |
| <I/σ(I)> | 24.7 | 1.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.4 | 10.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2M potassium phosphate dibasic, 20% PEG 3350 |
