6AEE
Crystal structure of the four Ig-like domains of LILRB1 complexed with HLA-G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97922 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.683, 154.681, 98.198 |
| Unit cell angles | 90.00, 102.24, 90.00 |
Refinement procedure
| Resolution | 45.420 - 3.303 |
| R-factor | 0.2227 |
| Rwork | 0.220 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2dyp 4ll9 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.631 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rpim | 0.105 | |
| Number of reflections | 29866 | 2993 |
| <I/σ(I)> | 7.89 | |
| Completeness [%] | 99.9 | |
| Redundancy | 4.2 | |
| CC(1/2) | 0.972 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.2M imidazole malate, 15%(w/v) PEG4000, pH 6.0 |
