6ACX

Crystal structure of Mycobacterium smegmatis Mfd in complex with ADP + Pi at 3.5 A resolution.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR-H
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-03-01
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	83.659, 160.440, 214.529
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.103 - 3.500
R-factor	0.2286
Rwork	0.226
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ac8
RMSD bond length	0.002
RMSD bond angle	0.524
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX ((1.11.1_2575: 000))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.730	3.660
High resolution limit [Å]	3.500	3.500
Rmerge	0.200	0.594
Rmeas	0.245	0.711
Rpim	0.104	0.305
Number of reflections	35989	4329
<I/σ(I)>	6.3	2.4
Completeness [%]	96.8	97.1
Redundancy	5.1	4.9
CC(1/2)	0.982	0.353

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	295	100mM HEPES sodium pH 7.2, 0.2M Na2So4, 20% PEG3350