6AAX
Crystal structure of TFB1M and h45 with SAM in homo sapiens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2016-03-16 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9790 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 104.120, 104.120, 449.378 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.627 - 2.994 |
| R-factor | 0.2245 |
| Rwork | 0.222 |
| R-free | 0.27060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gc5 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.208 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 6.460 | 3.000 |
| Rmerge | 0.222 | 0.063 | |
| Rmeas | 0.225 | 0.064 | |
| Rpim | 0.042 | 0.010 | 0.353 |
| Total number of observations | 1275274 | ||
| Number of reflections | 30398 | 3373 | 2947 |
| <I/σ(I)> | 4.1 | ||
| Completeness [%] | 100.0 | 100 | 100 |
| Redundancy | 42 | 37.6 | 43 |
| CC(1/2) | 1.000 | 0.814 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M potassium chloride, 0.025M magnesium sulfate hydrate, 0.05M HEPES sodium, pH 7.0, 20% v/v polyethylene glycol 200 |
