6AAX

Crystal structure of TFB1M and h45 with SAM in homo sapiens

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2016-03-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9790
Spacegroup name	P 61 2 2
Unit cell lengths	104.120, 104.120, 449.378
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.627 - 2.994
R-factor	0.2245
Rwork	0.222
R-free	0.27060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gc5
RMSD bond length	0.009
RMSD bond angle	1.208
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	3.110
High resolution limit [Å]	3.000	6.460	3.000
Rmerge	0.222	0.063
Rmeas	0.225	0.064
Rpim	0.042	0.010	0.353
Total number of observations	1275274
Number of reflections	30398	3373	2947
<I/σ(I)>	4.1
Completeness [%]	100.0	100	100
Redundancy	42	37.6	43
CC(1/2)		1.000	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2M potassium chloride, 0.025M magnesium sulfate hydrate, 0.05M HEPES sodium, pH 7.0, 20% v/v polyethylene glycol 200