6AAF
Crystal structure of fission yeast Atg8 complexed with the helical AIM of Hfl1.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2016-11-24 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 33.798, 108.866, 35.120 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.120 - 2.197 |
R-factor | 0.2161 |
Rwork | 0.211 |
R-free | 0.25930 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zpn |
RMSD bond length | 0.004 |
RMSD bond angle | 0.730 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.120 | 2.330 |
High resolution limit [Å] | 2.197 | 2.197 |
Rmerge | 0.122 | 1.331 |
Number of reflections | 7091 | 1101 |
<I/σ(I)> | 8.1 | 1.1 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 6.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 27.5% PEG8000, 0.2 M sodium acetate, 0.1 M Bis-Tris pH 5.5 |