6AAE

Crystal structure of Chloramphenicol-Metabolizaing Enzyme EstDL136

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-26
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 21 21 2
Unit cell lengths	118.943, 153.586, 44.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.258 - 1.641
R-factor	0.1899
Rwork	0.189
R-free	0.22010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u4n
RMSD bond length	0.007
RMSD bond angle	1.068
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.700
High resolution limit [Å]	1.640	1.640
Number of reflections	100067
<I/σ(I)>	23
Completeness [%]	100.0
Redundancy	14.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	500mM Ammonium fluoride (pH6.5), 30% PEG3350, 5% glycerol and 120mM TCEP