6AA0

Crystal Structure of Toxoplasma gondii Prolyl tRNA Synthetase (TgPRS) in Apo Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	300
Detector technology	PIXEL
Collection date	2017-11-18
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980066
Spacegroup name	P 31
Unit cell lengths	113.070, 113.070, 199.813
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	69.932 - 3.200
R-factor	0.1899
Rwork	0.188
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xif
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER (2.8.0)
Refinement software	PHENIX (1.15rc2_3428)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.910	3.280
High resolution limit [Å]	3.200	3.200
Number of reflections	47193	3515
<I/σ(I)>	10.3	2.7
Completeness [%]	100.0	100
Redundancy	10.8	10.7
CC(1/2)	0.996	0.844

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		300	Published