6A9W
Structure of the bifunctional DNA primase-polymerase from phage NrS-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-06-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 119.874, 57.570, 47.631 |
Unit cell angles | 90.00, 94.03, 90.00 |
Refinement procedure
Resolution | 38.540 - 1.800 |
R-factor | 0.1803 |
Rwork | 0.178 |
R-free | 0.21840 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 38.540 | 38.540 | 1.840 |
High resolution limit [Å] | 1.800 | 9.000 | 1.800 |
Rmerge | 0.077 | 0.051 | 1.042 |
Rmeas | 0.078 | 0.052 | 1.057 |
Rpim | 0.013 | 0.010 | 0.173 |
Number of reflections | 30021 | 212 | 1758 |
<I/σ(I)> | 32.8 | ||
Completeness [%] | 99.7 | 80.4 | 99.5 |
Redundancy | 36.8 | 29.8 | 36.8 |
CC(1/2) | 1.000 | 1.000 | 0.971 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100mM Bis-Tris, pH 6.5, 0.2M Li2SO4, 25% PEG 3350 |