6A9P
Crystal structure of the human glial fibrillary acidic protein 1B domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-06-08 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 |
Unit cell lengths | 23.588, 106.158, 113.225 |
Unit cell angles | 77.56, 90.14, 83.80 |
Refinement procedure
Resolution | 50.000 - 2.510 |
R-factor | 0.27071 |
Rwork | 0.269 |
R-free | 0.31086 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3uf1 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.229 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.062 | 0.614 |
Number of reflections | 29465 | |
<I/σ(I)> | 18.2 | |
Completeness [%] | 94.9 | |
Redundancy | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 100 mM Tris-HCl pH 8.0, 400 mM NaCl and 22% (w/v) PEG10,000 |