6A8K
Crystal structure of Ice-binding Protein from a Sea-Ice Microalga
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 95 |
Detector technology | PIXEL |
Collection date | 2015-06-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9800 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 36.054, 47.586, 134.517 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.590 - 1.400 |
R-factor | 0.13806 |
Rwork | 0.137 |
R-free | 0.16220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3vn3 |
RMSD bond length | 0.029 |
RMSD bond angle | 2.478 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.600 | 1.480 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.057 | 0.434 |
Number of reflections | 46394 | |
<I/σ(I)> | 24.1 | 6.3 |
Completeness [%] | 99.7 | 98.7 |
Redundancy | 12.3 | 11.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris-HCl pH8.5, 2.0M Ammonium dihydrogen phosphate |