6A8D

Crystal Structure of Chlamydomonas reinhardtii ARF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-11-22
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	43.818, 44.244, 113.481
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.740 - 2.340
R-factor	0.22251
Rwork	0.220
R-free	0.26563
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hur 1rrg 3aq4 5a1u
RMSD bond length	0.014
RMSD bond angle	1.991
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.750	2.430
High resolution limit [Å]	2.340	2.350
Rmeas	0.100	0.734
Rpim	0.029	0.259
Number of reflections	9681
<I/σ(I)>	35.71	2.44
Completeness [%]	98.8	92.8
Redundancy	10.7	5.9
CC(1/2)		0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	20% PEG8000, 200mM Ammonium Sulphate