6A7X
Rat Xanthine oxidoreductase, D428A variant, NAD bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-11-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 98.135, 137.792, 222.029 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.630 - 2.150 |
R-factor | 0.19807 |
Rwork | 0.195 |
R-free | 0.25488 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.339 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | EPMR |
Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.630 | 41.630 |
High resolution limit [Å] | 2.150 | 2.150 |
Number of reflections | 306011 | |
<I/σ(I)> | 1.72 | |
Completeness [%] | 99.4 | |
Redundancy | 1.72 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 293 | PEG 8000, HEPES, lithium sulfate, pH 6.2 |