6A7U
Crystal structure of histone H2A.Bbd-H2B dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 61.640, 141.910, 56.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.390 - 2.600 |
R-factor | 0.2367 |
Rwork | 0.235 |
R-free | 0.26290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3afa |
RMSD bond length | 0.010 |
RMSD bond angle | 1.429 |
Data reduction software | MOSFLM |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.390 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.094 | 0.696 |
Number of reflections | 8011 | 959 |
<I/σ(I)> | 10.7 | |
Completeness [%] | 100.0 | |
Redundancy | 6.3 | |
CC(1/2) | 0.998 | 0.901 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 289 | 0.1M sodium citrate tribasic dihydrate pH5.5 38% PEG 200 |