6A6M
Crystal structure of an outward-open nucleotide-bound state of the eukaryotic ABC multidrug transporter CmABCB1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 41 3 2 |
| Unit cell lengths | 174.340, 174.340, 174.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.620 - 1.900 |
| R-factor | 0.16747 |
| Rwork | 0.165 |
| R-free | 0.20764 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.537 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.620 | 1.950 |
| High resolution limit [Å] | 1.890 | 1.900 |
| Number of reflections | 72007 | |
| <I/σ(I)> | 13 | |
| Completeness [%] | 99.8 | |
| Redundancy | 21.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 21% PEG 2000 MME, 50 mM potassium nitrate and 50 mM magnesium nitrate |
